Synthetic antibody libraries help to design, construct, and successfully generate highly functional antibodies for various applications. Its characteristics of optimized framework sequences (a robust scaffold) and controlled CDRs diversity give it unique advantages compared to natural antibodies.
Synbio Technologies utilizes novel trimer oligonucleotide directing mutagenesis for codon optimization and synthesizing antibody CDR3 or all CDRs to create the variant regions scFv or nanobody libraries. The synthetic scFv antibody libraries can be applied to the research of improving the binding kinetics and affinity of studied antibodies. The advantages of nanobody libraries are higher solubility, improved stability in reducing conditions, as well as higher expression yield and thermal stability.