Polyclonal antibodies are a mixture of antibodies that recognize different epitopes of a specific antigen and can be produced by collecting antiserums after artificially immunizing the host. Because it can recognize multiple epitopes, it has a higher affinity and sensitivity than monoclonal antibodies. Polyclonal antibodies are also more tolerant to small antigen changes.
With rich experience in antigen design and mature antigen/antibody preparation technology, Synbio Technologies can provide one-stop customized services from antigen design, gene synthesis, antigen preparation, animal immunity, to high throughput antibody production. In addition, some host animals (such as mice, rabbits, goats, etc.) and various kinds of antigens (such as peptide antigens, protein antigens, small molecule antigens, etc.) are available. Our technical team will evaluate your requirements and tailor your project to meet your needs. Ultimately, we can deliver high quality polyclonal antibodies for your scientific research.
- High Titer: The titer of ELISA will be greater than 1:100000. For protein antigens, the delivered polyclonal antibodies will be positive for Western Blot.
- Customized Services: Provide exclusive experimental scheme for each customer to meet your personalized requirements.
- One-Stop Service: We can provide one-stop service from gene synthesis, antigen preparation, to high quality antibody production.
- Confidentiality: As your reliable partner, we will maintain a high degree of confidentiality for your sequences and products.
- Professional communication: We will maintain frequent progress updates and issue communications throughout the entire project.
|Antigen Preparation||Antigen provided by the customer:
1. The peptide 10 mg;
2. Recombinant protein 5~8mg, more than 85% purity;
3. Small molecular antigens;
|2-4 weeks||QC report of antigen preparation||Inquiry|
|Synthesized or prepared by Synbio Technologies:
1. Design and synthesize polypeptide, coupling with KLH/BSA/OVA;
2. Codon optimization, gene synthesis, recombinant protein expression and purification;
|Animal Immunization||Immunize 3 ~ 4 times||6~7 weeks||/|
|Antibody Purification||Antigen affinity purification or Protein A/G affinity purification||1-2 weeks||0.5-10 mg purified antibody, purity >80%|
|Quality Control||Antibody purity test (SDS-PAGE);
Specific detection of antigen and antibody (Western Blot analysis);
Antibody titer detection (ELISA, titer > 1:100000);
|1-2 weeks||QC report|
|Additional Tests||Antibody affinity test
Identification of antibody subtypes
Polyclonal antibody VS Monoclonal Antibody
|Polyclonal Antibody||Monoclonal Antibody|
|Identify multiple epitopes of a specific antigen||Identify a unique epitope of a specific antigen|
|More cross-reactions with similar antigens; Could amplify the signal of the target protein with low expression level; Higher staining background interference||Specific detection of an unique target epitope, without cross-reactions with other antigens; Low staining background interference|
|More tolerant to small antigen changes and can detect homologous protein families||Detection of an unique antigen or a single member of a protein family|
|Higher purity of the immunogen will obtain better polyclonal antibodies||Impure immunogens can also produce high purity antibodies|
|The quality of antibodies varies greatly from batch to batch||Easy to standardize, the antibody test results from different batches are stable and consistent|
|The production cost is lower and the turnaround time is shorter||The production cost is higher and the turnaround time is longer|