Monoclonal antibody sequencing includes hybridoma cell line sequencing and clonal B cell sequencing. Both of these kinds of cells can produce a single antibody against a unique antigen after screening. Synbio Technologies uses 5’RACE and Sanger sequencing technologies to provide both accurate and efficient hybridoma/clonal B cell sequencing services across a variety of species.
Standard antibody variant region PCR amplification primers design schematic
mRNA isolated from hybridoma samples is subjected to RT-PCR with unique species-specific primer sets to amplify the target regions. Synbio Technologies’s antibody variable region sequencing service has identified more than 10,000 hybridoma cell lines. More than 95% of the samples have been successfully sequenced with both the heavy chain and light chain identified. Even with technically difficult samples, Synbio Technologies’s approach has demonstrated a high success rate in numerous cases. Synbio Technologies’s service, coupled with online data tracking and management system, provides customers with absolute confidence at every step.
Following the successful cloning of variable regions, the sequences can be fused to a variety of IgG or IgM backbones. Alternatively, variable region sequences can be reformatted as scFv or bispecific antibodies in various configurations. Transiently produced antibodies can then be validated and characterized by downstream analytical services.
Cell Sample Preparation and shippping