After the fragments encoding Cas9 and sgRNA are transfected into cells through plasmids or other means, Cas9 cuts the target genome at a specific site, causing DNA double-strand break (DSB), which is joined through nonhomologous end joining (NHEJ) or homologous recombination (HR), inserts or deletes (indel) of DNA, or relies on a homologous template containing specific mutations for targeted modification of the target site. Synbio Technologies uses amplicon sequencing to test the results of CRISPR/Cas9 genome editing, as well as to analyze off-target effects, which helps researchers quickly locate gene mutation sites and study gene functions.

CRISPR Amplicon Sequencing
Competitive Advantages
- High Coverage: Each reaction can perform multiplex analysis of hundreds to thousands of amplicons.
- High Flexibility: It can be used for all kinds of mutation verification and screening genetic variation.
- Highly Customized: Analysis services can be customized.
- Cost-effective: Compared with whole-genome sequencing, it can reduce sequencing cost and turnaround time.
CRISPR Amplicon Sequencing Process

Service Specification
Amplicon Size | Platform Configuration | Price | Turnaround Time | Deliverables |
---|---|---|---|---|
70-280 bp | Illumina 2×150 bp | Quote | 3-4 weeks |
|
280-480 bp | Illumina 2×250 bp | 4-5 weeks |
* For more information please contact us at quote@synbio-tech.com.