After the fragments encoding Cas9 and sgRNA are transfected into cells through plasmids or other means, Cas9 cuts the target genome at a specific site, causing DNA double-strand break (DSB), which is joined through nonhomologous end joining (NHEJ) or homologous recombination (HR), inserts or deletes (indel) of DNA, or relies on a homologous template containing specific mutations for targeted modification of the target site. Synbio Technologies uses amplicon sequencing to test the results of CRISPR/Cas9 genome editing, as well as to analyze off-target effects, which helps researchers quickly locate gene mutation sites and study gene functions.

CRISPR Amplicon Sequencing

Competitive Advantages

  • High Coverage: Each reaction can perform multiplex analysis of hundreds to thousands of amplicons.
  • High Flexibility: It can be used for all kinds of mutation verification and screening genetic variation.
  • Highly Customized: Analysis services can be customized.
  • Cost-effective: Compared with whole-genome sequencing, it can reduce sequencing cost and turnaround time.

CRISPR Amplicon Sequencing Process

Service Specification

Amplicon Size Platform Configuration Price Turnaround Time Deliverables
70-280 bp Illumina 2×150 bp Quote 3-4 weeks
  • Sequencing chromatogram
  • Data analysis report
280-480 bp Illumina 2×250 bp 4-5 weeks

* For more information please contact us at

Related Services