The transfection of plasmids into mammalian cells directly incorporates genetic material into the genome to form a stable transfection. Stable transfection is used to create cell populations that will continue to express the genetic characteristics of the foreign material and then pass it on to the offspring. In order to achieve stable transfection, there should be selective pressure to force cells to integrate plasmid DNA into the genome.
Reporter genes are usually used to easily monitor the transfection efficiency and expression levels in cells. Synbio Technologies provides a variety of reporter gene expression vectors to meet the screening needs of different cell lines and efficiently screen expression according to cell-specific screening conditions.

Fluorescent Proteins, Promoters, and Selectable Markers Selection

Different promoters have different activation activities in different cells. For example, in hematopoietic stem cells, SFFV has much better activation activity than CMV. There are also differences in tissue-specific expression. AAT is highly expressed in liver tissues and weakly expressed in other tissues. hSyn is highly expressed in nerve cells and weakly expressed in other tissues. Synbio Technologies provide a variety of fluorescent proteins, promoters, and mammalian expression vectors with selectable markers to meet the needs of cell-specific screening.

Schematic diagram of mammalian cell expression vector

Selectable markers in mammalian cells work similarly to that in bacteria.
A table of the most commonly used selectable markers are listed below:

Name Gene Conferring Resistance Cell Types Mode of Action
Blasticidin bsd HEK293, CHO, HeLa, COS-1NIH3T3 Inhibits termination step of translation
G418/Geneticin neo HEK293, CHO,HeLa, NIH3T3, Jurkat T cells Blocks polypeptide synthesis at 80S; inhibits chain elongation
Hygromycin B hygB CHO, HeLa, NIH3T3, Jurkat T cells Blocks polypeptide synthesis at 80S; inhibits chain elongation
Puromycin pac HEK293, HeLa, Jurkat T cells Inhibits protein synthesis; premature chain termination
Zeocin Shbla HEK293, CHO, HeLa, NIH3T3, COS-1, Jurkat T cells Complexes with DNA; causes strand scissions

The promoters of mammalian expression vectors also have different applicable objects.
The most commonly used promoters are listed below:

Promoter Primarily used for RNA Transcript Description
CMV General expression mRNA Strong mammalian expression promoter from the human cytomegalovirus
EF1a General expression mRNA Strong mammalian expression from human elongation factor 1 alpha
SV40 General expression mRNA Mammalian expression promoter from the simian vacuolating virus 40
PGK1 (human or mouse) General expression mRNA Mammalian promoter from the phosphoglycerate kinase gene
Ubc General expression mRNA Mammalian promoter from the human ubiquitin C gene
Human beta actin General expression mRNA Mammalian promoter from the beta actin gene
CAG General expression mRNA Strong hybrid mammalian promoter
TRE General expression mRNA Tetracycline response element promoter
UAS General expression mRNA Drosophila promoter containing Gal4 binding sites
CaMKIIa Gene expression for optogenetics mRNA Ca2+/calmodulin-dependent protein kinase II promoter
H1 small RNA expression shRNA From the human polymerase III RNA promoter
U6 small RNA expression shRNA From the human U6 small nuclear promoter

* For more information please contact us at quote@synbio-tech.com.

Vector Types

Vector Name Vector Map Infection Effect Description
pMT1-CMV-Amp-NF-Neomycin Vector Type: Mammalian Cell Expression Vector Promoter: CMV Promoter Vector Resistance: Ampicillin Screening Marker: Neomycin
pMT2-CMV-Amp-mCherry-Neomycin Vector Type: Mammalian Cell Expression Vector Promoter: CMV Promoter Vector Resistance: Ampicillin Vector Tag: mCherry Screening Marker: Neomycin
pMT3-CMV-Amp-mCherry-Neomycin-N-Terminal 10xHis-TEV Vector Type: Mammalian Cell Expression Vector Promoter: CMV Promoter Vector Resistance: Ampicillin Vector Tags: mCherry Purification Tag: N-10xHis affinity tag-TEV Screening Marker: Neomycin
pMT4-CMV-Amp-mCherry-Neomycin-C-Terminal 10xHis-TEV Vector Type: Mammalian Cell Expression Vector Promoter: CMV Promoter Vector Resistance: Ampicillin Vector Tag: mCherry Purification Tag: C-10xHis affinity tag-TEV Screening Marker: Neomycin
pMT5-CMV-Amp-GFP-Neomycin-N-Terminal 10xHis-TEV Vector Type: Mammalian Cell Expression Vector Promoter: CMV Promoter Vector Resistance: Ampicillin Vector Tag: GFP Purification Tag: N-10xHis affinity tag-TEV Screening Marker: Neomycin
pMT6-CMV-Amp-GFP-Puromycin-N-Terminal 10xHis-TEV Vector Type: Mammalian Cell Expression Vector Promoter: CMV Promoter Vector Resistance: Ampicillin Vector Tag: GFP Purification Tag: N-10xHis affinity tag-TEV Screening Marker: Puromycin
pMT7-CMV-Amp-GFP-Puromycin-C-Terminal 10xHis-TEV Vector Type: Mammalian Cell Expression Vector Promoter: CMV Promoter Vector Resistance: Ampicillin Vector Tag: GFP Purification Tag: C-10xHis affinity tag-TEV Screening Marker: Puromycin
pMT8-CMV-Amp-YFP-Neomycin-N-Terminal 10xHis-TEV Vector Type: Mammalian Cell Expression Vector Promoter: CMV Promoter Vector Resistance: Ampicillin Vector Tag: YFP Purification Tag: C-10xHis affinity tag-TEV Screening Marker: Neomycin
pMT9-CMV-Amp-YFP-Blasticidin-N-Terminal 10xHis-TEV Vector Type: Mammalian Cell Expression Vector Promoter: CMV Promoter Vector Resistance: Ampicillin Vector Tag: YFP Purification Tag: C-10xHis affinity tag-TEV Screening Marker: Blasticidin
pMT10-CMV-Amp-YFP-Blasticidin-N-Terminal 3xStrepII-TEV. Vector Type: Mammalian Cell Expression Vector Promoter: CMV Promoter Vector Resistance: Ampicillin Vector Tag: YFP Purification Tag: N-10xHis affinity tag-TEV Screening Marker: Blasticidin
pMT11-CMV-Amp-Cerulean-Neomycin-N-Terminal 10xHis-TEV Vector Type: Mammalian Cell Expression Vector Promoter: CMV Promoter Vector Resistance: Ampicillin Vector Tag: Cerulean Purification Tag: N-10xHis affinity tag-TEV Screening Marker: Neomycin
pMT12-CMV-Amp-Cerulean-Zeocin-N-Terminal 10xHis-TEV Vector Type: Mammalian Cell Expression Vector Promoter: CMV Promoter Vector Resistance: Ampicillin Vector Tag : Cerulean Purification Tag: N-10xHis affinity tag-TEV Screening Marker: Zeocin
pMT13-CMV-Amp-Cerulean-Zeocin-N-3xStrepII-tag-TEV Vector Type: Mammalian Cell Expression Vector Promoter: CMV Promoter Vector Resistance: Ampicillin Vector Tag: Cerulean Purification Tag: N-3xStrepII tag-TEV Screening Marker: Zeocin
Vector Name Vector Map Description
pMT14-CMV-Kan Vector Type: Mammalian Cell Expression Vector
Promoter: CMV Promoter
Vector Resistance: Kan
pMT15-CMV-Kan-YFP Vector Type: Mammalian Cell Expression Vector
Promoter: CMV Promoter
Vector Resistance: Kan
Vector Tag: YFP
pMT16-CMV-Kan-YFP-Blasticidin Vector Type: Mammalian Cell Expression Vector
Promoter: CMV Promoter
Vector Resistance: Kan
Vector Tag: YFP
Screening Marker: Blasticidin
pMT17-CMV-Kan-mCherry Vector Type: Mammalian Cell Expression Vector
Promoter: CMV Promoter
Vector Resistance: Kan
Vector Tag: mCherry
pMT18-CMV-Kan-smURFP Vector Type: Mammalian Cell Expression Vector
Promoter: CMV Promoter
Vector Resistance: Kan
Vector Tag: smURFP
pMT19-CMV-Kan-smURFP-Hygromycin B Vector Type: Mammalian Cell Expression Vector
Promoter: CMV Promoter
Vector Resistance: Kan
Vector Tag: smURFP
Screening Marker: Hygromycin B
pMT20-CMV-Kan-Cerulean Vector Type: Mammalian Cell Expression Vector
Promoter: CMV Promoter
Vector Resistance: Kan
Vector Tag: Cerulean
Screening Marker: Hygromycin B
pMT21-CMV-Kan-Cerulean-Zeocin Vector Type: Mammalian Cell Expression Vector
Promoter: CMV Promoter
Vector Resistance: Kan
Vector Tag: Cerulean
Screening Marker: Zeocin
Vector Name Vector Map Description
pAb20-hCK human-antibody-expression-vector Vector Type: Human Antibody Expression Vector
Promoter: CMV Promoter
Vector Resistance: Ampicillin
Fc Type: hCK
pAb20-hCL-1 human-antibody-expression-vector-1 Vector Type: Human Antibody Expression Vector
Promoter: CMV Promoter
Vector Resistance: Ampicillin
Fc Type: hCL
pAb20-hCHIgG1 hCH IgG1 Vector Type: Human Antibody Expression Vector
Promoter: CMV Promoter
Vector Resistance: Ampicillin
Fc Type: hCH IgG1
pAb20-hCL-2 hCL Vector Type: Human Antibody Expression Vector
Promoter: CMV Promoter
Vector Resistance: Ampicillin
Fc Type: hCL
pAb20-hCHIgG2 hCH IgG2 Vector Type: Human Antibody Expression Vector
Promoter: CMV Promoter
Vector Resistance: Ampicillin
Fc Type: hCH IgG2
pAb20-hCHIgG3 hCH IgG3 Vector Type: Human Antibody Expression Vector
Promoter: CMV Promoter
Vector Resistance: Ampicillin
Fc Type: hCH IgG3
pAb20-hCHIgG4 hCH IgG4 Vector Type: Human Antibody Expression Vector
Promoter: CMV Promoter
Vector Resistance: Ampicillin
Fc Type: hCH IgG4
pAb20-hCHIgM hCH IgGM Vector Type: Human Antibody Expression Vector
Promoter: CMV Promoter
Vector Resistance: Ampicillin
Fc Type: hCH IgGM
Vector Name Vector Map Description
pAb20-mCK mCK Vector Type: Mouse Antibody Expression Vector
Promoter: CMV Promoter
Vector Resistance: Ampicillin
Fc Type: mCK
pAb20-mCL-1 pAb20-mCL-1 Vector Type: Mouse Antibody Expression Vector
Promoter: CMV Promoter
Vector Resistance: Ampicillin
Fc Type: mCL
pAb20-mCL-2 pAb20-mCL-2 Vector Type: Mouse Antibody Expression Vector
Promoter: CMV Promoter
Vector Resistance: Ampicillin
Fc Type: mCL
pAb20-mCHIgG1 pAb20-mCHIgG1 Vector Type: Mouse Antibody Expression Vector
Promoter: CMV Promoter
Vector Resistance: Ampicillin
Fc Type: mCH IgG1
pAb20-mCHIgG2a pAb20-mCHIgG2a Vector Type: Mouse Antibody Expression Vector
Promoter: CMV Promoter
Vector Resistance: Ampicillin
Fc Type:mCH IgG2a
pAb20-mCHIgG2b pAb20-mCHIgG2b Vector Type: Mouse Antibody Expression Vector
Promoter: CMV Promoter
Vector Resistance: Ampicillin
Fc Type:mCH IgG2b
pAb20-mCHIgG3 pAb20-mCHIgG3 Vector Type: Mouse Antibody Expression Vector
Promoter: CMV Promoter
Vector Resistance: Ampicillin
Fc Type:mCH IgG3
pAb20-mCHIgM pAb20-mCHIgM Vector Type: Mouse Antibody Expression Vector
Promoter: CMV Promoter
Vector Resistance: Ampicillin
Fc Type:mCHIgM

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