Protein A/Protein G/Protein L: The Ultimate Solutions for Antibody Purification
Welcome to Synbio Technologies, your premier source for high-quality Protein A, Protein G, and Protein L products. Our special functional proteins are engineered to be secreted and expressed on the surface of bacteria, providing specific binding capabilities to immunoglobulins. With their unique properties and versatility, these proteins offer unparalleled advantages in various applications.
Protein A, also known as Staphylococcal protein A (SPA), Protein G, or Streptococcal protein G (SPG), and Protein L, commonly referred to as Peptostreptococcus protein L (PPL), are widely recognized immunoglobulin binding proteins. They exhibit diverse binding modes and profiles, enabling efficient binding to a broad range of mammalian immunoglobulins. When combined, these three proteins offer complementary advantages and flexibly adapt to different purification requirements.
At Synbio Technologies, we produce Protein A/Protein G/Protein L products using our state-of-the-art E. coli expression system. Our products are meticulously crafted to ensure high specificity and affinity. By reducing non-specific protein binding, our Protein A/Protein G/Protein L solutions enable the production of purified antibodies suitable for a wide array of applications.
Benefits of our Protein A/Protein G/Protein L products:
- Antibody Purification: Our proteins provide effective purification solutions for antibodies, minimizing non-specific binding and optimizing purification yields. Whether you are working on small-scale research or large-scale production, our products deliver consistent and reliable results.
- Versatile Applications: The high specificity and affinity of our Protein A/Protein G/Protein L products make them ideal for immunoprecipitation and co-immunoprecipitation analysis. With their exceptional binding capabilities, they facilitate precise isolation and analysis of target proteins, opening new avenues for scientific discovery.
- Customized Solutions: We understand that each project has unique requirements. That’s why we offer customized solutions tailored to your specific needs. Our team of experts is ready to assist you in selecting the most suitable Protein A/Protein G/Protein L product and optimizing its use in your applications.
- Exceptional Quality: At Synbio Technologies, quality is our top priority. We adhere to stringent manufacturing processes and rigorous quality control measures to ensure the highest standards of our Protein A/Protein G/Protein L products. You can trust us to provide reliable, reproducible, and high-performance solutions.
Unlock the full potential of your antibody purification and analysis with our advanced and innovative solutions. Contact us today to discuss your specific requirements and embark on a journey of scientific excellence.
Related
| Species | Protein A | Protein G | Protein L | Species | Protein A | Protein G | Protein L | ||
|---|---|---|---|---|---|---|---|---|---|
| Human | lgG | +++ | +++ | +++ | Sheep | lgG | + | ++ | – |
| lgG1 | ++++ | ++++ | ++++ | lgG1 | + | ++ | – | ||
| lgG2 | ++++ | ++++ | ++++ | lgG2 | +++ | +++ | – | ||
| lgG3 | – | +++ | +++ | Dog | ++ | + | NT | ||
| lgG4 | ++++ | ++++ | ++++ | Pig | +++ | ++ | +++ | ||
| lgA | + | – | +++ | Rat | lgG | + | ++ | +++ | |
| lgA1 | + | – | +++ | lgG1 | – | + | +++ | ||
| lgA2 | + | – | +++ | lgG2a | – | ++++ | +++ | ||
| lgD | – | – | +++ | lgG2b | – | ++ | +++ | ||
| lgE | ++ | – | +++ | lgG2c | ++ | ++ | +++ | ||
| lgM | + | – | +++ | lgG3 | + | ++ | NT | ||
| Rabbit | lgG | +++ | +++ | + | Mouse | lgG | ++ | ++ | +++ |
| Cow | lgG | + | +++ | – | lgG1 | + | ++++ | +++ | |
| lgG1 | + | +++ | – | lgG2a | ++++ | ++++ | +++ | ||
| lgG2 | +++ | +++ | – | lgG2b | +++ | +++ | +++ | ||
| Cat | lgG | +++ | + | NT | lgG3 | ++ | +++ | +++ | |
| Horse | lgG | ++ | ++++ | NT | lgM | – | – | +++ | |
| Goat | lgG | + | ++ | – | Chicken | lgY | – | – | – |
| lgG1 | + | +++ | – | Monkey | lgG | ++++ | ++++ | NT | |
| lgG2 | +++ | +++ | – | Hamster | + | ++ | +++ | ||
| Guinea-pig | lgG1 | ++ | + | NT | Koala | – | + | NT | |
| lgG2 | ++ | + | NT | Llama | – | + | NT | ||
| Strong binding (++), medium interaction (+), weak or no interaction (-), not test (NT) | |||||||||
At Synbio Technologies, we pride ourselves on delivering exceptional service and providing you with the most advanced solutions for your antibody purification needs. Our Protein A, Protein G, and Protein L products come with a range of service advantages that set us apart from the rest.
Low Endotoxin Levels: We understand the importance of minimizing endotoxin contamination in your research. That’s why our Protein A/Protein G/Protein L products undergo rigorous testing using the Limulus Amoebocyte Lysate (LAL) Assay, ensuring that the endotoxin levels are below 100 EU/mg. With our low endotoxin products, you can have confidence in the integrity of your experiments.
High Purity Guarantee: Quality matters, and we prioritize delivering products of the highest purity. Our Protein A/Protein G/Protein L solutions undergo stringent quality control measures, including Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE) analysis. With purity levels exceeding 95%, you can trust that our products will deliver consistent and reliable results.
Flexible Product Forms: We understand that different research needs call for different product forms. Our Protein A/Protein G/Protein L products are available in both liquid and lyophilized powder forms, allowing you to choose the option that best suits your experimental requirements. Whether you prefer the convenience of a liquid solution or the long-term stability of a lyophilized powder, we have you covered.
Readily Available: We know that time is of the essence in research. That’s why we maintain a well-stocked inventory of our Protein A/Protein G/Protein L products, ensuring quick and efficient delivery to our customers. With multiple sizes available, you can easily find the right quantity for your specific needs, saving you time and streamlining your workflow.
Fig1. Protein A electrophoresis diagram
Sequence information:4× (ADNKFNKEQXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEAKKLNDAQAPK)-KCKHHHHHH
Recombinant Protein A is obtained from an E. coli expression system and consists of 4 directional evolution highly specific high-affinity IgG-binding structural domains arranged in tandem with a strong specific affinity for the Fc fragment of IgG. The recombinant protein Protein A is coupled to the substrate as an affinity ligand and can specifically bind to antibody molecules in the sample while allowing other heteroproteins to flow through, with extremely high selectivity.
Fig 2. Protein G electrophoresis diagram
Sequence information:2× (TYKLVINGKTLKGXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXDDATKTFTVTE)-KCKHHHHHH
Recombinant Protein G is obtained from an E. coli expression system and contains 2 directional evolution highly specific high-affinity IgG binding regions of SPG. To ensure maximum specific binding of IgG, the cell wall binding region, cell membrane binding region and albumin binding region have been removed from recombinant Protein G, reducing cross-reactivity and non-specific binding. Recombinant Protein G is widely used as a ligand coupled with resin for purification of polyclonal or monoclonal IgG antibodies.
Fig 3. Protein L electrophoresis diagram
Sequence information:4× (PKEEVTIKAQLIXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXFAGKEKTPEE)-KCKHHHHHH
Recombinant Protein L is obtained from an E. coli expression system and contains 4 directional evolution highly specific, high-affinity kappa-binding domains. It has a unique binding by kappa light chain interaction without interfering with the antibody-antigen binding site. The cell wall binding region, cell membrane binding region and albumin binding region have been removed from the recombinant Protein L to ensure maximum IgG specific binding.
