Nuclear magnetic resonance (NMR) is a key analytical technique for a variety of discovery and analytical applications including small-molecule screening, protein-protein interactions and bio-equivalence. Synbio Technologies offers high quality, customized NMR services including for researchers in life science and pharmaceutical industry. These services include NMR resonance assignments and 3D structures of proteins and complexes. With these services, combined with our advanced molecular biology platforms, Synbio Technologies can handle our customers’ projects starting from gene synthesis all the way to crystallization and structure determination.

Service Procedure

  1. Preparation of stable isotope labeled protein sample
    Preparation of protein samples with 15N-, 15N13C-, 2H15N13C-, 2H15N13C-ILV-, 19F- specific labeling.
  2. 1D/2D NMR
    1D/2D NMR can be used to assess sample quality, purity and stability. NMR screening can also be used to identify interacting ligands and protein partners.
  3. Rotational Correlation Time Measurements
    Rotational correlation time is the time it takes a molecule to rotate one radian and it depends on the molecule size. Application includes: estimation of molecular weight (e.g., oligomerization) and, more recently, to detect presence of antigen in sample in liquid biopsy.
  4. Small Molecule Screening
    NMR can used to detect small molecule interactions with target proteins using either ligand-detected methods including saturation transfer difference (STD) and WaterLogsy, or protein-detected chemical shift perturbation studies. Ligand screening can then be done with high throughput techniques by using a micro NMR probe (30 uL sample volume) and automated NMR sample changer.
  5. Protein NMR Resonance Assignments
    The process describes the correlation of NMR chemical shifts with specific atoms in a protein or other biomolecules. Resonance assignments enable analysis of protein structures and dynamics, as well as studies of ligand binding.
  6. Protein Dynamics Studies
    NMR can be used to give insights into protein dynamics, entropic changes due to complex formation, and allosteric effects associated with ligand binding.
  7. Protein Interaction Studies
    Chemical shift perturbation and/or X-filtered NOESY experiments can be used to detect protein-protein or protein-ligand interactions.
  8. Nucleic Acid NMR
    NMR can be used to probe nucleic acid structure and interactions.