Prokaryotic expression is mature and easy to be operated, but when it comes to the proteins of eukaryotic organism, error folding is a frequent occurrence since glycosylation and folding partner are involved. Fortunately, the expression system of pichia pastorisis is complementary to this situation to a certain extent. Relying on powerful synthetic biology technology platform and industry-leading codon optimization software NGTM Codon, Synbio Technologies provides you high-quality and considerate yeast expression protein services with more than 10 years’ experience in protein expression and purification, which can effectively save your cost and time.

Service Specifications

Steps Description Deliverables Turnaround Time Price
Gene Synthesis  (optional)
  • Codon optimization
  • Gene synthesis
  • Vector construction (pPICZα, pPIC9, pGAPZα…)
  • 2-5 μg lyophilized plasmid DNA
  • 1 tube of glycerol bacteria/puncture bacteria
  • Sequencing Validation Report
 Starting from 10 BDs Quote
Electrotransformation and Screening Positive Clones (PCR method)
  • Linearized the plasmid and transformed into pichia pastoris cells to screen positive clones
  • Screening report of positive clones
 2-3 weeks Starting from $3,499
Pilot Protein Expression
  • Three positive clones will be used to verify the expression, then perform expression analysis validation (WB)
  • Optimal expression strain
 2-3 weeks
Protein Expression and Purification
  • 1 L or larger scale expression
  • One or more steps purification
  • Purity will be detected by SDS-PAGE or Western blot (optional)
  • Purified protein products
  • Certificate of analysis (COA)
 3-4 weeks

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