Baculovirus insect cell system offers several unique advantages over E. coli expression system, such as post-translational modifications, higher yield for secreted protein, and proved solubility. It is appropriate for expressing proteins that maybe harmful to mammalian cells, such as kinases and toxic proteins. With years of experience in protein expression, Synbio Technologies possesses abundant experience in baculovirus Insect cell protein expression, particularly the expression of toxic proteins, kinases, large proteins, membrane protein and intracellular proteins.

Insect System Advantages

Proprietary NG Codon Optimization Technology: Significantly improves protein expression levels and success rate.
Multiple Purification Strategies: Affinity chromatography, ion exchange, hydrophobic chromatography, gel filtration, and more, provides diversified choice of protein purities.
Comprehensive Protein Identification Services: SDS-PAGE, Western Blot, MALDI-TOF, and HPLC

Service Specifications

Service Steps Description Deliverables Turnaround Time Price
Gene Synthesis (optional)
  • 2-5 μg lyophilized plasmid DNA
  • 1 tube of glycerol/bacteria/puncture bacteria
  • Sequencing Validation Report
Starting from 10 BDs Quote
Baculovirus preparation
  • Generation of recombinant Bacmid DNA
  • Generation of P1 stock, P2 stock and determination of virus titer
  • Experiment report
3-5 weeks Starting from $2,699
Validation and analysis of protein expression
  • P2 generation virus infect SF9 cells
  • Expression analysis validation (WB)
  • Ni column affinity purification
  • A small amount of Protein
  • Expression evaluation report
2-4 weeks
Protein Expression and Purification
  • 1 L or larger scale expression
  • Purified protein products
  • Certificate of analysis (COA)
1-2 weeks

* The delivery time of this step bay vary due to the length and/or difficulties of the sequence(s). Please contact our project manager for a quote.