Untargeted metabolomics refers to the non-biased detection of the dynamic changes of all small molecule metabolites in cells, tissues, organs, or organisms after stimulation or disturbance by LC-MS, GC-MS, and NMR technology. Differential metabolites are screened by bioinformatics analysis, and then pathway analysis is performed to reveal the physiological mechanism of their changes.
- Phenotypic and Physiological Functions
- Pathological Study of Diseases
- Clinical Diagnosis and Treatment
- Drug Research
- Food Science and Nutrition
- Animal Husbandry and Agroforestry
- Environmental Toxicology
- Advanced Instrument Platform: UHPLC-QE-MS & UHPLC-QTOF-MS & GC-Q-MS.
- Abundant Commercial Databases: Many commercial databases, more than 2000 standard databases, more accurate qualitative data.
- Strict Quality Control: Rigorous method validation in analytical processes.
- Personalized Service: Professional bioinformatics teams, Personalized bioinformatics analysis services.
Untargeted Metabolomics Work Flow
Untargeted metabolomics is used to detect and quantify total metabolites in biological samples. The design idea of untargeted metabolomics experiments is to compare differential metabolites under different treatments or physiological states and to explore the difference of mechanisms. At least 8 biological replicates are required for cell, plant, and microbial samples in each group. At least 10 biological replicates are required for animal samples in each group. At least 30 biological replicates are required for clinical samples each group.