Metabolomics reflects static metabolite abundance, and the increase of a single metabolite may be due to either the activation of the synthetic pathway or the inhibition of the consumption pathway. Therefore, Metabolomics is often not enough to explain all the problems in the study of specific metabolic pathways and metabolic networks. As a complementary tool to metabolomics, Metabolic Flux Analysis (MFA) uses stable isotopes (13C or 15N) to label specific molecules and track their metabolic processes in an organism to obtain dynamic information about metabolites in metabolic pathways. MFA is the only tool that can quantify energy flows (i.e., ATP, cofactor generation and consumption) and their balances.


  • Medical Research: Tumor metabolism mechanism, immune related diseases, metabolic diseases, stem cell development and differentiation, etc.
  • Agriculture and Forestry: Simulate physiological processes of biology, bacterial metabolites, stress resistance, commercial crop quality, breeding, animal nutrition, meat quality, etc.

Competitive Advantages

  • Advanced Instrument Platform: Orbitrap LC-MS & UHPLC-QQQ-MS & GC-Q-MS.
  • Personalized Service: Professional bioinformatics teams & personalized bioinformatics analysis services.

MFA Work Flow

Service Specifications

Service Types Sample Requirements Platform Price Turnaround Time Deliverables
Glycolysis Flux Ratio
  • Cell≥4×106
  • Stored in liquid nitrogen or -80℃
  • Transportation by dry ice
  • Orbitrap LC-MS
  • GC-Q-MS
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  • Raw data
  • Data analysis report
TCA Pathways Flux
Pentose Phosphate Pathways

Analysis Item

Number Analysis Item Number Analysis Item
1 Raw data preprocessing 5 13C flux ratio differential analysis
2 Isotopic distribution of metabolites 6 Hierarchical cluster analysis
3 Identification of metabolic pathways 7 PCA analysis
4 13C flux ratio analysis

Data Analysis

Isotopic Information of Metabolites

13C Flux Ratio Differential Analysis

Hierarchical Cluster Analysis

PCA Analysis