The genomes of microorganisms usually contain thousands of genes. It is particularly important to find the correlation between these genes and phenotypes with high throughput and low-cost technologies. The genome-wide CRISPR interference or activation library can analyze the associated gene groups of a certain phenotype under a variety of screening conditions, providing a powerful tool for the study of microbial cell factories.
Synbio Technologies provides efficient and accurate microbial genome editing services. Our E. coli genome CRISPR interference library, yeast genome interference library, and yeast genome activation library products effectively shorten the library delivery cycle.
- Syno E. coli Genome CRISPR Interference Library
Our Syno E. coli genome CRISPR interference library includes 55,671 sgRNAs and 400 control (non-targeted) sgRNAs. Based on strict off-target quality control, at least one sgRNA targets 98.6% of the protein coding genes (4,140) and 79.8% of the RNA coding genes (178) in E. coli. At least three sgRNAs target 96.8% of protein coding genes and 48.9% of RNA coding genes. The Syno E. coli genome CRISPR interference library has significantly higher gene coverage at the genome level than Tn-seq with a similar library size. It can study the relationship between thousands of genes and specific phenotypes in bacteria at one time.
- Syno Yeast Genome CRISPR Interference and Activation Libraries
The design principle and number of sgRNA in our Syno yeast genome CRISPR interference libraries and Syno yeast genome CRISPR activation libraries are the same: First, when the transcription start site is known, the target is selected from 220 nt upstream to 20 nt downstream of the transcription start. Second, when no transcription start site is available, a target between 350 and 30 nt upstream of the coding sequence is selected. Based on these rules, 61,094 sgRNAs are designed for all annotated protein-coding genes, excepting those predicted open reading frames characterized as “dubious”, and also against non-coding RNAs. Most genes are targeted by 10 unique sgRNA. The main difference between the two libraries is the type of vector used. The interference library uses pdCas9_ Mix_ Yeast, and the activation library uses pdCas9_ VPR_ Yeast.
- Accurate Design: sgRNA sequences with high target activity and low off-target rate are developed with sgRNA design models and deep learning algorithms.
- Cost Efficiencies: CRISPR libraries with high editing efficiency reduce research cost by eliminating the need for additional screening later.
- Strict Quality Control: Coverage of the constructed libraries are >99.9% with <10 uniformity.
- Comprehensive Services: We provide customized services from sgRNA synthesis, library construction, NGS validation, virus packaging to bioinformatics analysis.
||Syno E. coli Genome CRISPR Interference Library
||55,671 sgRNA sequences and 400 control (non-targeted) sgRNA sequences
||Syno Yeast Genome CRISPR Interference Library
||61,094 sgRNA sequences
||Syno Yeast Genome CRISPR Activation Library
||61,094 sgRNA sequences
sgRNA Customized Design
sgRNA Library Construction
Human Gemone CRISPR Knockout Libraries
Microbial Genome Editing
Mammalian Genome Editing
sgRNA Bioinformatics Analysis
1. Tianmin Wang, Changge Guan, Jiahui Guo, et al. Pooled CRISPR interference screening enables genome-scale functional genomics study in bacteria with superior performance. Nat Commun. 2018; 9: 2475.
2. Nicholas J. McGlincy, Zuriah A. Meacham, Kendra K. Reynaud, et al. A genome-scale CRISPR interference guide library enables comprehensive phenotypic profiling in yeast. BMC Genomics. 2021; 22: 205.
3. Elena Cámara, Ibai Lenitz, and Yvonne Nygård. A CRISPR activation and interference toolkit for industrial Saccharomyces cerevisiae strain KE6-12. Sci Rep. 2020; 10: 14605.