CRISPR-Cas9 sgRNA Library

CRISPR-Cas9/gRNA (CRISPR-Cas RNA-Guided nuclease) is considered as the biggest breakthrough in genome editing technology after TALEN and ZFN. It precisely edits DNA through the RNA-directed Cas9 nuclease. Previous studies indicated that the on-target gene knockout rates were higher than that with TALEN and ZFN. Besides, the construction of sgRNA-Cas9 is easier and more convenient. It has been widely applied to animal and plant breeding, directed evolution, and the site-specific restoration of genetic diseases. Synbio Technologies offers the designing, construction and validation of CRISPR-Cas9 gene target system through our patent pending platform in a highly cost and time-effective approach. We offer one stop solution for CRISPR Cas9 projects, including genome scale gene targeting.

Competitive Advantages of CRISPR-Cas9

  • Applicable in any type of cell lines
  • Simultaneously knockout multiple targets
  • More efficient
  • Fast turnaround time

Services Specifications

  • CRISPR-Cas9-based primer design
  • CRISPR-Cas9-based plasmid construction
  • CRISPR-Cas9-based target locus validation
  • CRISPR-Cas9-based stable cell line construction
  • CRISPR-Cas9-based stem cell construction
  • Certificate of analysis (COA) for project
  • Lyophilized plasmid DNA (2~5 µg), or libraries
  • Sequencing chromatogram
  • QC report
Service Type Price Turnaround Time
(Business Days)
CRISPR-Cas9-based primer design Inquiry
CRISPR-Cas9-based plasmid construction
CRISPR-Cas9-based target locus validation Inquiry
CRISPR-Cas9-based stable cell line construction
CRISPR-Cas9-based stem cell construction


  • CRISPR-Cas9-based gene knock-out
  • CRISPR-Cas9-based gene knock-in
  • CRISPR-Cas9-based transcription activation
  • CRISPR-Cas9-based transcription inhibition