Stability: RNA molecules are inherently unstable and prone to degradation by nucleases. Protecting RNA from degradation during synthesis, purification, and storage is crucial to maintain its integrity and functionality.
Purity: Contaminants, such as residual DNA, chemicals, or other impurities, can affect the quality and activity of synthesized RNA. Thorough purification methods must be employed to remove these impurities and ensure the desired purity of the RNA molecule.
Yield: Obtaining high yields of RNA, especially for longer sequences, can be challenging. Low yields can limit the amount of RNA available for downstream applications and may require additional optimization steps to improve efficiency.
Chemical modifications: Introducing chemical modifications to RNA molecules can enhance their stability, specificity, or other properties. However, these modifications may impact the efficiency and fidelity of RNA synthesis, requiring careful optimization and evaluation.
Bioactivity: RNA molecules must possess the correct folding and secondary/tertiary structure to be biologically active. Ensuring proper folding and functional RNA structure during synthesis is essential for their effectiveness in interacting with other molecules and carrying out their intended functions.
Sequence fidelity: Errors during RNA synthesis, such as mismatches or truncations, can affect the accuracy and fidelity of the RNA sequence. Maintaining high sequence fidelity is crucial, particularly for applications requiring precise RNA sequences.
Addressing these challenges often requires optimization of synthesis protocols, purification methods, and quality control measures. Advancements in RNA synthesis technologies and methodologies continue to tackle these challenges and improve the overall quality, yield, and bioactivity of synthesized RNA molecules.
Synbio Technologies offers efficient and customizable RNA products and services to meet the diverse needs of researchers. Leveraging their expertise in synthetic biology, they consider various factors such as codon preference, mRNA secondary structure, GC content, and translation efficiency to optimize RNA sequences. They utilize advanced bioinformatics and deep learning algorithms to design sequences that result in improved protein expression content, solubility, and overall performance.
Furthermore, Synbio Technologies provides tailored synthesis services for RNA molecules of different lengths, specifications, modifications, and labeling options. This flexibility enables researchers to obtain high-quality RNA products for gene function analysis and the development of therapeutic strategies.
The advancements in RNA synthesis facilitated by companies like Synbio Technologies hold tremendous potential for precision medicine. The ability to rapidly and efficiently produce RNA molecules with specific sequences enables the development of personalized therapies and diagnostics. By customizing treatments based on an individual’s genetic and molecular characteristics, precision medicine offers more targeted and effective approaches to patient care.
As the field of RNA synthesis continues to evolve, it is expected to play an increasingly critical role in driving advancements in precision medicine and revolutionizing the way diseases are diagnosed and treated.
 Beck J D, Reidenbach D, Salomon N, et al. mRNA therapeutics in cancer immunotherapy[J]. Molecular cancer, 2021, 20(1): 1-24. Geall A J, Mandl C W, Ulmer J B. RNA: the new revolution in nucleic acid vaccines[C]//Seminars in immunology. Academic Press, 2013, 25(2): 152-159. Shin H, Park S J, Yim Y, et al. Recent advances in RNA therapeutics and RNA delivery systems based on nano particles[J]. Advanced Therapeutics, 2018, 1(7): 1800065.