Gene synthesis was first successfully conducted in 1972 on a yeast tRNA by Har Gobind Khrona and associates.Since 1972, the technology and mechanisms of gene synthesis have flourished in order to successfully obtain the genetic sequence specific to a wide range of research topics.Gene synthesis offers many major advantages over the traditional gene cloning methods: molecular cloning and polymerase chain reaction (PCR).These two traditional methods have two major restrictions,the first is that these methods are only capable of gene amplification and cloning, as opposed to gene synthesis.The other restriction molecular cloning and polymerase chain reaction have is that the preexisting genetic sequence of interest must be obtained and then amplified.The important advantage gene synthesis offers is that the genetic code you wish to study does not need to be physically obtained in order to be amplified.The technology currently allows us to synthesize “de novo” sequences.This means that a sequence of interest,whether it is viral DNA or a cancer cell’s mutation in a specific gene, can be synthesized without the physical copy of the gene being present.Without the physical copy of the gene of interest being needed,the necessary amount of time and money is drastically reduced when compared with traditional methods of cloning, amplification,targeted mutagenesis,and gene target knockouts.Due to this advantage gene synthesis has been becoming more and more popular in various fields of research.

Due to the ease of use and cost effective mechanism, gene synthesis has been used countless amounts of times in varying fields of scientific research. Gene synthesis has most notably and recently been used to conduct viral research in order to produce safer, more effective DNA vaccines. It has also been used to better understand mechanisms for cancer cell metabolic regulation. Gene synthesis is currently being used for targeted gene therapy, and other topics of interest that seemed almost impossible 30 years ago. In addition to the research that has already been conducted, gene synthesis offers a myriad of possibilities of application ranging from gene regulation to better understanding evolution and antibiotic resistance. Gene synthesis makes it possible to build variant libraries, genes, operons, increase the function of proteins, and even test all the orthologs of a particular gene.

The advantages gene synthesis offers are seemingly endless.The main advantage that gene synthesis has to offer is the ease of synthetically manipulating or cloning a gene of interest with or without the physical copy of the gene itself.Synbio Technologies offers, through our Syno®DNA Platform, an extremely accurate, time efficient, and cost effective mechanism to analyze your sequence of interest and successfully synthesize the sequence.All sequences constructed by Synbio Technologies are verified using Sanger sequencing, and are guaranteed to be 100 percent identical to your sequence of interest.In addition to the high quality synthesized sequences, Synbio Technologies offers competitive prices on gene synthesis for a wide range of gene lengths up to 100 kb.With the competitive prices and accuracy, on top of the additional money and resources needed for PCR and molecular cloning, it is clear that gene synthesis is the more effective mechanism to utilize when conducting certain types of genetic research.