In the mysterious world of the life sciences, gene sequences are the key to open the door to the unknown. Gene sequences can be split into two different categories: simple sequences and complex sequences.

The increasing trend of utilizing complex sequences in life science research is surging like never before! This is not only the inevitable product of scientific progress, but also the result of many other factors. These intricate factors have paved the way for genetic research, and each step is full of new opportunities and discoveries.

• Sequence Length: Synthesizing gene sequences of thousands of base pairs requires ensuring that fragments are correctly synthesized and accurately spliced.
• Base Composition: DNA sequences with extremely high or very low GC content are prone to sequence breakage or mismatch during synthesis.
• Complex Structure: Complex structure in the sequence may lead to sequence breakage or mismatch during the synthesis process.
• Repetitive Sequences: Synthesis of repetitive sequences in long sequences leads to error-prone accumulation during synthesis.
• Host Cell Stability: Specific elements or structures in the sequences lead to unstable gene expression in the host cell.
• Technology Optimization: Gene synthesis systems require constant optimization and innovation. Oligonucleotide synthesis and sequencing technologies are also in need of constant updating.

Synbio Technologies’ gene synthesis platform provides you with fast, high-quality and efficient gene synthesis services. With over a decade of synthesis experience combined with a variety of AI-enhanced analysis tools, we can synthesize even the most complex and long sequences. Whether it is a complex sequence design or a complicated synthesis process, we will provide you with the best solution in the industry. Let Synbio Technologies do the heavy lifting while you focus on your research!

References

[1] Ellis T, Adie T, Baldwin GS. DNA assembly for synthetic biology: from parts to pathways and beyond. Integr Biol (Camb). 2011 Feb;3(2):109-18.
[2] Kosuri S, Church GM. Large-scale de novo DNA synthesis: technologies and applications. Nat Methods. 2014 May;11(5):499-507.
[3] Andrianantoandro E, Basu S, Karig DK, Weiss R. Synthetic biology: new engineering rules for an emerging discipline. Mol Syst Biol 2006; 2: 2006.0028.