Single B cell antibody technologies offer novel mechanisms for the discovery of naturally occurring antibody drugs. This is accomplished by combining high throughput techniques with low sifting rates and avoiding drawbacks of unnatural pairing from in vitro screening. Synbio Technologies provides single B cell antibody services including the development and preparation of B cell antibodies, B cell antibody sequencing and deep analysis, etc.

B cells, also known as B lymphocytes, mainly in charge of humoral immunity of human bodies and present function under antigenic stimulation. When an adaptive immune response is warranted, activated B cells proliferate and undergo somatic hypermutation of their B cell receptor (BCR) to form diverse cell clonings. Additionally, as a complex molecule, antibodies have three complementarity determining regions (CDRs) in each heavy (VH) and light (VL) chains. Moreover, the CDR3 of the heavy chain is most abundantly altered and tends to perform at a high level of hypermutation at the junction between the V, D and J gene regions. Therefore, the natural VH/VL antibodies produced by B cells are critical for understanding antibody biology and design.

Synbio Technologies’ GenoTM Ab Platform provides professional single B-cell antibody sequencing analysis services to our customers. These services aid our customers to better assess the diversity of their immune system of interest as well as the relationship between the generated antibody libraries and diseases. Both aspects have large implications in the field of antibody research and development.

Featured Advantages

  • Internal antibody database platform to score the antibody’s heavy and light chain pairs to screen the most logical intact antibody sequence
  • Unique in-house data mining tools to pre-process antibody sequencing to minimize sequencing errors such as false positives
  • Experienced research team with complete bioinformatics analysis technology to mine high-value antibody information

Service Process

Synbio Technologies Case Study

Synbio Technologies’ Bioinformatics team analyzed various antibody chrematistics based on the client’s original antibody sequencing data. These characteristics included: the VDJ recombination, V/J gene frequency, frequencies of V-J pairing, CDR3 length analysis, B cell clone count, receptor diversity analysis, intergroup diversity difference analysis, cloning and expression of CDR3 lengths. The final professional research reports were provided.

1) Frequency of VDJ gene usage analysis

2) Analysis of CDR3 region length

3) Analysis of CDR3 kinds

CDR3 Reads Column 3 Column 4
DNNNDMR 3560 0.004869 7
VISRTL 1688 0.002308 6
AVDSNYQLI 899 0.001229 9
AVNRDDKII 718 0.000982 9
AATDSWGKLQ 696 0.000952 10
AVTDSWGKLQ 627 0.000857 10
AVNTGNQFY 595 0.000814 9
AVTGGGNKLT 573 0.000784 10
AVPTGTASKLT 562 0.000769 11
LVGDTGRRALT 540 0.000738 11
AEITLT 517 0.000707 6

4) Ordering of total length sequence

Amino Acid Gene Length Acid Amino Length Abundance
1491 147 0.096324
20 139 0.044324
540 143 0.040395
529 145 0.041238
516 147 0.041955
504 143 0.042773
496 141 0.043976
441 145 0.040898
433 145 0.041868
387 148 0.039055

5) Frequency analysis of VH gene difference between different samples

6) Saturation analysis of sequencing

7) Diversity analysis of cloning

TCR/BCR Shannon Entropy Inverse Simpson
BCR 19.7458 340.758

Service Specifications

Service Detail Analysis Solftware TAT (Business Days) Deliverable
Single B cell Antibody Sequencing Analysis Data mining of sequencing, Antibody information analysis etc. R, Python Software etc. 7-10 Analysis report