Phage display is a widely used technique for the study of protein–protein, protein–peptide, and protein–DNA interactions. With the development of antibody drugs and vaccines, phage display libraries have a revolutionized impact on antibody engineering. It makes it possible for screening and identifying billions of target-binding proteins in a short time.
Synbio Technologies utilizes an M13 single-stranded filamentary phage display system, making the recombinant proteins or antibodies co-express with the phage pIII protein. With the help of helper phages, the proteins or antibodies will participate in the phage assembly and be displayed on the phage surface to form a phage display library. After several rounds of affinity screening with specific target molecules, the one which can specifically bind to target molecules will be enriched and obtain the corresponding DNA information by sequencing.
Synbio Technologies’s phage display technology has been successfully applied in synthetic scFv antibody library construction. We also can provide flexible and efficient customized services according to the special needs of customers.