Eukaryotic RNA sequencing is based on Next Generation Sequencing (NGS) and provides comprehensive and rapid access to all transcript sets of a particular cell or tissue in certain state. It is used to study gene structure and function, alternative splicing and prediction of new transcript, etc. Eukaryotic RNA sequencing can be divided into reference transcriptome and non-reference transcriptome according to with or without reference genomes.
Eukaryotic non-reference RNA sequencing is the sequencing of all RNA transcribed by specific tissues or cells of eukaryotes in a specific situation; Eukaryotic reference RNA sequencing is the sequencing of all mRNA transcribed by specific tissues or cells of eukaryotes in a specific situation. Compared with the reference genome, it can not only comprehensively and rapidly analyze the mRNA sequence and abundance information, but also the gene structure and the new transcript.
High Quality: With the advanced HiSeq 4000 sequencing platform, high-quality s data can be read quickly and efficiently.
High Coverage: Digital signals directly measure the sequence of almost all transcript fragments.
Stand-specific Library Construction: Use dUTP stand-specific library construction proposal to ensure the directionality and accurate quantitative results of transcripts.
Whole Transcriptome Analysis: Specific probes or genomic information are not necessary, we can directly perform the most comprehensive transcriptome analysis on any species.
Correlation Analysis: Combining multiple data groups, carrying out cross-group correlation analysis.
Eukaryotic RNA Sequencing
Cell, tissue, serum, plasma, total RNA, etc.
HiSeq 4000, PE150
Total RNA ≥ 2g (minimum 1g)
Concentration ≥ 50 ng/μL