A transcriptome refers to the sets of all transcription products in a cell under a certain physiological condition, including mRNA, rRNA, tRNA, and non-coding RNA. In short, it means the set of all mRNAs. The research scope of transcriptomes is all mRNA of a particular cell in certain state. Based on Synbio Technologies’s high-throughput sequencing technology, almost all RNA information of a tissue or organ can be sequenced comprehensively. Eukaryotic and prokaryotic RNA sequencing is used to discover expressed genes in cells, tissues, or individuals under different physiological or pathological conditions. A transcriptome bonds a genome’s genetic information and biological functions. Nowadays, RNA sequencing is widely applied to a wide variety of biological research as well as clinical diagnosis and drug development.
The design idea of a transcriptome experiment is to compare different genes, and the common type is to compare the experimental group and the control group. With time and space factors considered, multiple comparative analyses can be implemented according to different growth stages or the occurrence and development of diseases. At least 3 biological replicates are required for each group.
1. Prokaryotic transcriptome sequencing
2. Eukaryotic transcriptome sequencing with reference genome
3. Eukaryotic transcriptome sequencing without reference genome