shRNA design is typically divided into two formats: the simple stem-loop shRNA and the microRNA-adapted shRNA (Fig.1). Due to the hairpin structure, shRNA is difficult to sequence in gene synthesis. Universal sequencing protocols can hardly work, especially for the second structure’s sequence (Fig.2). However, Synbio Technologies has developed and enhanced the protocol, and obtained high quality sequencing results (Fig.3). With upgraded process and long-standing expertise, Synbio Technologies is always exceptionally good at dealing with difficult structures such as hairpin structure, poly structure, etc..