Since the birth of synthetic biology in 2000, metabolic engineering and synthetic biology research have made increasingly huge progress on high value-added natural products. The synthesis and construction of metabolic pathways as well as diverse DNA assembly technologies play fundamental roles in microbiology, biochemistry, and many other relevant fields.

Based on Syno 2.0 and Syno 3.0 gene synthesis tools and strong long DNA assembly capability, Synbio Technologies is confident in providing professional technical support for metabolic pathway synthesis and library assembly. Specific services include metabolic pathway synthesis and customized metabolic pathway library assembly.

• Our Syno 2.0 platform is able to provide a fast synthesis of gene fragments and modular genes.
• Our Syno 3.0 high throughput synthesis platform can assure large-scale reserve of promoters, nucleic acid binding sites, terminators and so on.
• Reliable synthesis of long DNA; a single metabolic pathway has been synthesized to 20Kb.
• Wide range of library assembly technologies, allowing for customized library storage.

In this  project, the variety of promoters used was key to achieving a wide range of outcomes in the final library we constructed. We aimed for a library with the capacity to produce 100 different variations, and by ensuring each variation was represented 100 times, we successfully created 10,000 individual clones. When we tested these clones using PCR, a staggering 78% of them showed the desired results, and every single one was confirmed to be accurate.